PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis

PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis

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The blood-tumor barrier (BTB) restricts the efficient delivery of anti-glioma drugs to cranial glioma tissues.Increased BTB permeability may allow greater delivery of the therapeutic agents.Increasing evidence has revealed that PIWI proteins and PIWI-interacting RNAs (piRNAs) play an important role in tumor progression.However, whether PIWI proteins and piRNAs regulate BTB permeability remains unclear.In the present study, we demonstrated that the gauze fabric by the bolt PIWIL1/piRNA-DQ593109 (piR-DQ593109) complex was the predominant regulator of BTB permeability.

Briefly, PIWIL1 was upregulated in glioma endothelial cells (GECs).Furthermore, piR-DQ593109 was also overexpressed in GECs, as revealed via a piRNA microarray.Downregulation of PIWIL1 or piR-DQ593109 increased the permeability of the BTB.Moreover, PIWIL1 and piR-DQ593109, which formed a piRNA-induced silencing complex, sound anchor adj2 degraded the long non-coding RNA maternally expressed 3 (MEG3) in a sequenced-dependent manner.Furthermore, restoring MEG3 released post-transcriptional inhibition of Runt related transcription factor 3 (RUNX3) by sponging miR-330-5p.

In addition, RUNX3 bounded to the promoter regions and reduced the promoter activities of ZO-1, occludin, and claudin-5, which significantly impaired the expression levels of ZO-1, occludin, and claudin-5.In conclusion, downregulating PIWIL1 and piR-DQ593109 increased BTB permeability through the MEG3/miR-330-5p/RUNX3 axis.These data may provide insight into glioma treatment.